Effects of Eco-Enzyme Concentrations and Growing Media on the Growth Performance of Pakcoy Microgreens

Authors

  • Ni Kadek Desy Andya Dewi Agrotechnology Study Program, Faculty of Agriculture, Science and Technology, Warmadewa University, Denpasar, Bali, Indonesia
  • Adellia Novianti Study Program of Agrotechnology, Faculty of Agriculture, Science and Technology, Warmadewa University
  • I Gusti Bagus Udayana Study Program of Agrotechnology, Faculty of Agriculture, Science and Technology, Warmadewa University
  • I Nengah Muliarta Study Program of Agrotechnology, Faculty of Agriculture, Science and Technology, Warmadewa University
  • Ni Luh Putu Sulis Dewi Damayanti Study Program of Agrotechnology, Faculty of Agriculture, Science and Technology, Warmadewa University

DOI:

https://doi.org/10.22225/seas.10.1.14317.74-81

Keywords:

Microgreens; Pakcoy; Eco-Enzyme; Growing Media; Plant Growth

Abstract

Microgreens are young vegetable seedlings valued for their high nutritional content and rapid growth cycle. Pakcoy microgreens (Brassica rapa chinensis L.) have strong potential for sustainable urban agriculture. This study aimed to evaluate the effects of different eco-enzyme concentrations and growing media on the growth performance of pakcoy microgreens. The experiment was conducted using a factorial completely randomized design with two factors: eco-enzyme concentration and growing media type. Several growth parameters were observed, including plant height, number of leaves, fresh weight, and overall growth vigor. The results showed that both eco-enzyme concentration and growing media not  significantly influenced the growth of pakcoy microgreens. Higher eco-enzyme concentrations generally promoted better vegetative growth, particularly when combined with suitable growing media. The interaction between eco-enzyme application and growing media played an important role in optimizing microgreen growth performance. These findings indicate that eco-enzyme can be utilized as an environmentally friendly input to enhance pakcoy microgreen production. The use of appropriate growing media further improves growth outcomes, supporting sustainable and efficient microgreen cultivation systems. This study provides practical insights for small-scale growers and urban farmers seeking to improve microgreen productivity using eco-friendly alternatives.

References

[1] Toydemir G, Gultekin Subasi B, Hall RD, Beekwilder J, Boyacioglu D, Capanoglu E. Effect of food processing on antioxidants, their bioavailability and potential relevance to human health. Food Chem X. 2022;14:100334. DOI: 10.1016/j.fochx.2022.100334

[2] Bhaswant M, Shanmugam DK, Miyazawa T, Abe C, Miyazawa T. Molecules and health benefits. Molecules. 2023;28(867):1–24. DOI: 10.3390/molecules28040867

[3] Kariasa IGK, Nengah IM, Yuli MSY. Microgreen as a minimalist vegetable cultivation innovation in supporting family food security in urban areas. Indones J Agric Environ Anal. 2024;3(1):13–22.

[4] Singh A, Singh J, Kaur S, Gunjal M, Kaur J, Nanda V, et al. Emergence of microgreens as a valuable food, current understanding of their market and consumer perception: A review. Food Chem X. 2024;23:101527. DOI: 10.1016/j.fochx.2024.101527

[5] Charloq C. Analysis of bioactive components of pakcoy microgreens (Brassica rapa L.) on variations of planting media. J Agron Tanam Trop. 2024;6(2).

[6] Zhang X, Yang H, Zhou X, Xu J, Chen J, Liao L, et al. Potential benefits of vitamin A and its derivatives in glaucoma. Eur J Med Res. 2025;30(1). DOI: 10.1186/s40001-025-01923-6

[7] Bara?ska D, Panek J, Ró?alska S, Turnau K, Fr?c M. Microgreens as the future of urban horticulture and superfoods, supported by post-harvest innovations for shelf-life increase: A review. Sci Hortic. 2025;350:113321. DOI: 10.1016/j.scienta.2024.113321

[8] Bayisa YM, Bullo TA, Demissie TA, Sime CH, Fante KA, Gebryu AT, et al. Sustainable production of liquid organic fertilizer from solid waste composting via microwave-assisted for smart irrigation. Discov Agric. 2025;3(1). DOI: 10.1007/s44279-025-00041-9

[9] Fadlilla T, Budiastuti MTS, Rosariastuti MR. Potential of fruit and vegetable waste as eco-enzyme fertilizer for plants. J Penelit Pendidik IPA. 2023;9(4):2191–2200.

[10] Seth T, Mishra GP, Chattopadhyay A, Deb Roy P, Devi M, Sahu A, et al. Microgreens: Functional food for nutrition and dietary diversification. Plants. 2025;14(4):1–29. DOI: 10.3390/plants14040638.

[11] Suwegiono, Dalimunthe BA, Adam DH, Sitanggang KD. The effect of various planting media on the growth and yield of microgreen mustard (Brassica juncea L.). J Agron Tanam Trop. 2025;7(1):5–8.

[12] Balik S, Dasgan HY, Ikiz B, Gruda NS. The performance of growing-media-shaped microgreens: Growth, yield, and nutrient profiles of broccoli, red beet, and black radish. Horticulturae. 2024;10(12). DOI: 10.3390/horticulturae10121304

[13] Sukewijaya IM, Dwiyani R, Bimantara PO. Optimization of growing media to support microgreens growth and nutritional profile. Agro Bali. 2025;8(1):102–113.

[14] Lone JK, Pandey R, Gayacharan. Microgreens on the rise: Expanding our horizons from farm to fork. Heliyon. 2024;10(4):e25870.DOI: 10.1016/j.heliyon.2024.e25870

[15] Rachman IA, Umasugi B, Aji K, Hakim NFA, Sofyan A, Hasan ADA. Effect of eco-enzyme application on soil nutrient and plant productivity of green mustard–peanut in inceptisol. Kultivasi. 2025;24(2):196–205.

[16] Azuhro V, Dzakiy MA, Minarti IB. Effectiveness of eco enzyme for inducing flower growth in Dendrobium Sonia-ersakul orchids. J Pijar MIPA. 2024;19(1):179–184.

[17] Kern J, et al. Composts in growing media: What’s new and what’s next? Acta Hortic. 2021;32(3):167–186. DOI: 10.17660/ActaHortic.2021.1321.21

[18] Rachman IA, Umasugi B, Aji K, Hakim NFA, Sofyan A, Hasan ADA. Kultivasi. 2025. Effect of eco-enzyme application on soil nutrient and plant productivity of green mustard–peanut in inceptisol. 24(2): 196–205.

[19] Istiqomah A. Effect of growing media composition and nutrient solution on growth and yield of pakcoy (Brassica rapa L. Chinensis) in hydroponic substrate. Plantropica J Agric Sci. 2016;1(1):6–11.

[20] Partap M, Sharma D, Dahiya HN, Thakur M, Verma V, Ujala, et al. Microgreen: A tiny plant with superfood potential. J Funct Foods. 2023;107:105697. DOI: 10.1016/j.jff.2023.105697

[21] Sembiring SDBJ, Ginting N, Umar S, Ginting S. Effect of eco-enzyme concentration on growth and production of butterfly pea (Clitoria ternatea L.) as animal feed. J Peternak Integr. 2021;9(1):36–46.

[22] Bilalis D, Stavropoulos P, Mavroeidis A, Kourempes S, Kourempe MC, Roussis I, et al. Effect of substrate on microgreens production. Bull Univ Agric Sci Vet Med Cluj-Napoca Hortic. 2025;82(1):32–36. DOI: 10.15835/buasvmcn-hort:2025.0004

[23] Sekhurwane M, Tóth B, Moloi MJ. Effectiveness of soil, foliar, and seed selenium applications in modulating physio-biochemical and yield responses to drought stress in vegetable soybean (Glycine max L. Merrill). Plants. 2025;14(21):1–23. DOI: 10.3390/plants14213521

[24] Rawat M, Kumar A, Kumar S, Kaur R. Unlocking the potential of microgreens: Non-thermal innovations to enhance phytochemical and nutritional benefits. Food Chem X. 2025;29:102747. DOI: 10.1016/j.fochx.2025.102747

[25] Kesmayanti N, Purwanto RJ, Romza E, Kalsum U, Irmawati W, Putri K. Effectiveness of eco-enzyme for increasing growth and production of shallot from vegetative seeds. J Agric. 2025;4(2):106–114.

[26] Aguiar-Macedo M, Dulyanska Y, Guiné RPF, Costa DVTA, Redondo LM. PEF priming of seeds for microgreen production: A comparative study. Foods. 2025;14(1):1–26. DOI: 10.3390/foods14010123

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Published

2026-04-30

Issue

Vol. 10 No. 1 (2026)

Section

Articles

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