Distribution of Hepatitis B Virus Genotypes Among Patients at Internal Medicine Unit, Dr. Soetomo General Hospital, Surabaya
AbstractBackground: Hepatitis B virus (HBV) infection is a major health problem worldwide, especially in developing countries. The study of HBV genotypes is important to find out the diversity of HBV genotypes related to the severity of the disease, response to therapy, and clinical symptoms. Objective: This study was aimed to detect HBV genotypes in patients at Hepatology Outpatient Clinic, Dr. Soetomo Hospital Surabaya. Methods: This study was conducted on new patients at Hepatology Outpatient Clinic of Dr. Soetomo General Hospital, Surabaya in one month. Nested PCR was performed by targetting HBV surface genes. Samples with positive HBV DNA were sequenced and analysed further. Results: In this study, a total of 27 samples were obtained. The prevalence of HBV infection shown by positive HBsAg in patients with symptoms of liver disease was 55.55% (15/27 patients). Based on the results of electrophoresis from PCR products, positive HBV DNA was obtained in these 15 patients (100%). After sequencing samples with positive HBV DNA, genotype B of Indonesian strain was found to be predominant genotype (100%). Subgenotype analysis showed that 7/15 samples had B3 subgenotype (46.67%). Conclusion: In patients at Hepatology Outpatient Clinic of Dr. Soetomo General Hospital, Surabaya, the prevalence of HBV infection was high (55.55%) and genotype B was predominant. In Surabaya, HBV genotype infection still remained like the previous pattern, although in Indonesia there have been many inter-island and ethnic migration. Further similar studies are needed to obtain the diversity of other HBV genotypes.
World Health Organization. Hepatitis B Fact Sheet [Internet]. 2017 [cited 2017 Jan 8]. Available from: http://www.who.int/mediacentre/factsheets/fs204/en/
Center for Disease Control and Prevention. Hepatitis B FAQs for the Public [Internet]. 2016. Available from: https://www.cdc.gov/hepatitis/hbv/hbvfaq.htm#overview
Utsumi T, Yano Y, Lusida MI, Amin M, Soetjipto, Hotta H, et al. Serologic and molecular characteristics of hepatitis B virus among school children in East Java, Indonesia. Am J Trop Med Hyg. 2010;83(1):189–93.
Sunbul M. Hepatitis B virus genotypes: global distribution and clinical importance. World J Gastroenterol [Internet]. 2014 May 14 [cited 2014 Nov 26];20(18):5427–34. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=4017058&tool=pmcentrez&rendertype=abstract
Utama A, Octavia TI, Dhenni R, Miskad UA, Yusuf I, Tai S. Hepatitis B virus genotypes/subgenotypes in voluntary blood donors in Makassar, South Sulawesi, Indonesia. Virol J [Internet]. 2009;6:128. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2732614&tool=pmcentrez&rendertype=abstract
Thedja M. Genetic Diversity of Hepatitis B Virus in Indonesia: Epidemiological and Clinical Significance [Internet]. 2012. Available from: http://dspace.library.uu.nl/bitstream/handle/1874/257026/thedja.pdf?sequence=2. 2012
Biswas A, Panigrahi R, Pal M, Chakraborty S, Bhattacharya P, Chakrabarti S, et al. Shift in the hepatitis B virus genotype distribution in the last decade among the HBV carriers from eastern India: Possible effects on the disease status and hbv epidemiology. J Med Virol. 2013;85(8):1340–7.
Sunbul M. Hepatitis B virus genotypes: Global distribution and clinical importance. World J Gastroenterol. 2014;20(18):5427–34.
Pourkarim MR, Amini-Bavil-Olyaee S, Kurbanov F, Van Ranst M, Tacke F. Molecular identification of hepatitis B virus genotypes/subgenotypes: Revised classification hurdles and updated resolutions. World J Gastroenterol [Internet]. 2014;20(23):7152–68. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24966586%5Cnhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC4064061%5Cnhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=4064061&tool=pmcentrez&rendertype=abstract
Lindh M, Andersson a S, Gusdal a. Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus--large-scale analysis using a new genotyping method. J Infect Dis. 1997;175(6):1285–93.
Telenta PF, Poggio GP, López JL, Gonzalez J, Lemberg a, Campos RH. Increased prevalence of genotype F hepatitis B virus isolates in Buenos Aires, Argentina. J Clin Microbiol [Internet]. 1997;35(7):1873–5. Available from: http://www.ncbi.nlm.nih.gov/pubmed/9196213
Kemenkes RI. Riskesdas 2007 [Internet]. 2007. Available from: www.litbang.depkes.go.id
Robert C, Hustead TR, Ali SM, Pantsari MW. Mildly Elevated Liver Transaminase Levels: Causes and Evaluation. Am Fam Physician. 2017;96(11):709–15.
Giannini EG, Testa R, Savarino V. Liver enzyme alteration: a guide for clinicians. CMAJ. 2005;172(3):367–79.
Quint WG, Heijtink RA, Schirm J, Gerlich WH, Niesters HG.
Reliability of methods for hepatitis B virus DNA detection. J Clin Microbiol [Internet]. 1995;33(1):225–8. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=227915&tool=pmcentrez&rendertype=abstract
Xibing G, Xiaojuan Y, Juanhua W, Zhong H. Relationship Between HBV Genotypes B, C and Follicular Helper T Cells in Patients with Chronic Hepatitis B and its Significance. Hepat Mon [Internet]. 2013;13(1). Available from: http://www.hepatmon.com/?page=article&article_id=6221
Siburian MD, Utama A, Dhenni R, Arnelis N, Fanany I, Intan MDB, et al. High prevalence of hepatitis B virus genotype C/C1 in the Minangkabau ethnic group in Indonesia. Virol J [Internet]. 2013 Jan [cited 2014 Nov 27];10(1):27. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3583810&tool=pmcentrez&rendertype=abstract
Qian Z, Jianqiong W, Hongmei L, Rong Z, Li L, Jinping Z, et al. Distribution and Epidemiologic Trends of HBV Genotypes and Subtypes in 14 Countries Neighboring China. Hepat Mon [Internet]. 2015;15(5). Available from: http://www.hepatmon.com/?page=article&article_id=24422
Thedja MD, Muljono DH, Le SI, Sidarta E, Turyadi, Verhoef J, et al. Genogeography and immune epitope characteristics of hepatitis b virus genotype c reveals two distinct types: Asian and papua-pacific. PLoS One. 2015;10(7):1–17.
UNICEF. Indonesia Migration Profiles [Internet]. 2013. Available from: https://esa.un.org/miggmgprofiles/indicators/files/Indonesia.pdf
Ananta A, Utami DRWW, Handayani NB. Statistics on Ethnic Diversity in the Land of Papua, Indonesia. Asia Pacific Policy Stud [Internet]. 2016;3(3):458–74. Available from: http://doi.wiley.com/10.1002/app5.143
Shi W, Zhu C, Zheng W, Carr MJ, Higgins DG, Zhang Z. Subgenotype reclassification of genotype B hepatitis B virus. BMC Gastroenterol [Internet]. 2012;12(1):116. Available from: http://bmcgastroenterol.biomedcentral.com/articles/10.1186/1471-230X-12-116
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